Metabolism of Methanol in Plant Cells. Carbon-13 Nuclear Magnetic Resonance Studies

摘要

Using ¹³C-NMR, we demonstrate that [¹³C]methanol readily entered sycamore (Acer pseudoplatanus L.) cells to be slowly metabolized to [3-¹³C]serine, [¹³CH3]methionine, and [¹³CH3]phosphatidylcholine. We conclude that the assimilation of [¹³C]methanol occurs through the formation of ¹³CH3H4Pte-glutamate (Glu)n and S-adenosyl-methionine, because feeding plant cells with [3-¹³CH3]serine, the direct precursor of ¹³CH2H4Pte-Glun, can perfectly mimic [¹³CH3]methanol for folate-mediated single-carbon metabolism. On the other hand, the metabolism of [¹³C]methanol in plant cells revealed assimilation of label into a new cellular product that was identified as [¹³CH3]methyl-β-d-glucopyranoside. The de novo synthesis of methyl-β-d-glucopyranoside induced by methanol did not require the formation of ¹³CH3H4Pte-Glun and was very likely catalyzed by a “transglycosylation” process.