AB209630 a long non-coding RNA decreased expression in hypopharyngeal squamous cell carcinoma influences proliferation invasion metastasis and survival

摘要

Long noncoding RNAs (lncRNAs) are associated with the development, progression, and prognosis of human cancers. However, the clinical significance and biological function of lncRNAs in hypopharyngeal squamous cell carcinoma (HSCC) remain largely unknown. We characterized the novel lncRNA in vivo and in vitro. First, using qRT-PCR, we evaluated whether levels differ between HSCC tissues/cell lines and adjacent normal tissues/cell lines. We then assessed whether expression levels stimulate or inhibit proliferation, invasion, apoptosis, and metastasis in vitro. Finally, we investigated whether levels in tumor tissues were associated with survival outcomes. Our results demonstrated that levels were markedly lower in HSCC tissues and cells than in normal tissues and cells, and increased expression of level significantly inhibited growth, metastasis, and invasion and stimulated apoptosis in vitro. In addition, patients with decreased expression of had a significantly poorer prognosis than those with high expression. These data suggest that increased expression of might either stimulate or inhibit biological activities involved in HSCC development, indicating a potential application of in future treatment for this disease. This study suggest that functions as a tumor suppressor in HSCC, and its decreased expression may help predict a poor prognostic outcome of HSCC. Our future work will focus on the mechanisms of whether and how as a tumor suppressor gene is involved in HSCC development.