Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses V. Isolation of Additional Hybrids Which Differ in Their Simian Virus 40-Specific Biological Properties

摘要

Four new nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses have been isolated. Although these viruses (designated Ad2⁺ND2, Ad2⁺ND3, Ad2⁺ND4, and Ad2⁺ND5) were clonal derivatives of the same Ad2-SV40 hybrid population, they differ significantly from each other and from the previously isolated nondefective hybrid, Ad2⁺ND1, in their biological properties or in the amount of SV40-specific RNA induced during lytic infection.Like Ad2⁺ND1, Ad2⁺ND2, and Ad2⁺ND4 pass serially in both human embryonic kidney (HEK) and primary African green monkey kidney cells. In contrast, Ad2⁺ND3 and Ad2⁺ND5 pass serially only in HEK cells. Ad2⁺ND2 is like Ad2⁺ND1 in that it induces the SV40 U antigen, but not SV40 T antigen; however, in contrast to the perinuclear SV40 antigen induced by Ad2⁺ND1, the SV40 antigen induced by Ad2⁺ND2 is located peripherally in the cytoplasm as well as in the perinuclear region of infected cells. Ad2⁺ND4 induces both the SV40 T and U antigens. Ad2⁺ND3 and Ad2⁺ND₅ do not induce serologically detectable SV40 antigens and are distinguished from each other on the basis of the relative quantities of SV40-specific RNA which they induce. The induction of different SV40-specific functions suggests the incorporation of different segments of SV40 DNA within the genomes of the respective hybrid viruses.