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Analyses of Candida Cdc13 Orthologues Revealed a Novel OB Fold Dimer Arrangement Dimerization-Assisted DNA Binding and Substantial Structural Differences between Cdc13 and RPA70

机译:念珠菌Cdc13直系同源物的分析揭示了新型的OB折叠二聚体排列二聚化辅助DNA结合以及Cdc13和RPA70之间的实质性结构差异

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摘要

The budding yeast Cdc13-Stn1-Ten1 complex is crucial for telomere protection and has been proposed to resemble the RPA complex structurally and functionally. The Cdc13 homologues in Candida species are unusually small and lack two conserved domains previously implicated in telomere regulation, thus raising interesting questions concerning the mechanisms and evolution of these proteins. In this report, we show that the unusually small Cdc13 homologue in Candida albicans is indeed a regulator of telomere lengths and that it associates with telomere DNA in vivo. We demonstrated high-affinity telomere DNA binding by C. tropicalis Cdc13 (CtCdc13) and found that dimerization of this protein through its OB4 domain is important for high-affinity DNA binding. Interestingly, CtCdc13-DNA complex formation appears to involve primarily recognition of multiple copies of a six-nucleotide element (GGATGT) that is shared by many Candida telomere repeats. We also determined the crystal structure of the OB4 domain of C. glabrata Cdc13, which revealed a novel mechanism of OB fold dimerization. The structure also exhibits marked differences to the C-terminal OB fold of RPA70, thus arguing against a close evolutionary kinship between these two proteins. Our findings provide new insights on the mechanisms and evolution of a critical telomere end binding protein.
机译:发芽的酵母Cdc13-Stn1-Ten1复合物对于端粒保护至关重要,并且已提出在结构和功能上类似于RPA复合物。念珠菌属物种中的Cdc13同源物非常小,并且缺少两个以前涉及端粒调控的保守域,因此引起了有关这些蛋白质的机制和进化的有趣问题。在此报告中,我们显示了白色念珠菌中异常小的Cdc13同源物确实是端粒长度的调节剂,并且它在体内与端粒DNA相关。我们证明了热带假丝酵母Cdc13(CtCdc13)的高亲和力端粒DNA结合,并发现该蛋白通过其OB4结构域的二聚化对于高亲和力DNA结合很重要。有趣的是,CtCdc13-DNA复合物的形成似乎主要涉及许多假丝酵母端粒重复序列共有的六个核苷酸元件(GGATGT)的多个拷贝的识别。我们还确定了C. glabrata Cdc13的OB4结构域的晶体结构,揭示了OB折叠二聚化的新机制。该结构还对RPA70的C端OB折叠表现出显着差异,因此认为这两种蛋白之间存在紧密的进化亲缘关系。我们的发现为关键的端粒末端结合蛋白的机制和进化提供了新的见解。

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