A novel method for quantifying traction forces on hexagonal micropatterned protein features on deformable poly-dimethyl siloxane sheets

摘要

class="kwd-title">Method name: HexForce traction force measurement class="kwd-title">Keywords: Cell traction forces, Microcontact printing, Polydimethylsiloxane, Finite element analysis class="head no_bottom_margin" id="abs0010title">AbstractMany methods exist for quantifying cellular traction forces, including traction force microscopy and microfabricated post arrays. However, these methodologies have limitations, including a requirement to remove cells to determine undeflected particle locations and the inability to quantify forces of cells with low cytoskeletal stiffness, respectively. Here we present a novel method of traction force quantification that eliminates both of these limitations. Through the use of a hexagonal pattern of microcontact-printed protein spots, a novel computational algorithm, and thin surfaces of polydimethyl siloxane (PDMS) blends, we demonstrate a system that: class="first-line-outdent" id="lis0005">

• quantifies cellular forces on a homogeneous surface that is stable and easily manufactured.

• utilizes hexagonal patterns of protein spots and computational geometry to quantify cellular forces without need for cell removal.

• quantifies cellular forces in cells with low cytoskeletal rigidity.