AB184. Effects of target gene expression on ex vivo differentiated mesenchymal stromal cells transfected by lent viral vector with PDE5 of short hairpin RNA

摘要

IntroductionIt has been shown that the major causes of erectile dysfunction (ED) associated with aging were via a diffuse and progressive corporal fibrosis to cavernous smooth muscle cells (CSMCs) in the penis. Previous studies have demonstrated that bone marrow-mesenchymal stromal cells (BM-MSCs) could possess the ability for self-renewal, multiline age differentiation and in vivo functional regeneration of damaged tissues. It was suggested that they be induced to differentiate into smooth muscle-like cells in vitro and have a potential source for stem-cell-based gene therapy of ED. Specific phosphodiesterases (PDE5) has been proved to play an important role in regulating penile erection to return to flaccidity by hydrolysis of cyclic guanosine monophosphate (cGMP) as a specific hydratase. Therefore, inhibiting specifically the catalytic activity of the target gene PDE5 has been indicated to promote the accumulation of cGMP and enhance erection. In recent years, the introduction of short hairpin RNA (shRNA) has proved to be a powerful tool for suppressing strongly gene specific expression through a process known as RNA interference. This study was to describe that stem cell-based replacement therapy with rat BM-MSCs by transfected the lentiviral vector carrying silencing target gene PDE5 of short hairpin RNA (PDE5-shRNA) could aid in the regeneration of penile smooth musculature and might help to benefit recovery of erectile function.