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Genomic Insights into Stutzerimonas kunmingensis TFRC-KFRI-1 Isolated from Manila Clam (Ruditapes philippinarum): Functional and Phylogenetic Analysis

机译:从马尼拉蛤蜊 (Ruditapes philippinarum) 中分离的 Stutzerimonas kunmingensis TFRC-KFRI-1 的基因组学见解:功能和系统发育分析

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摘要

Stutzerimonas kunmingensis TFRC-KFRI-1, isolated from the gut of Manila Clam in the sediment of the West Sea of Korea, was investigated for its potential as a probiotic bacterium. This strain, belonging to the family Pseudomonadaceae, was previously classified as Pseudomonas kunmingensis but later reclassified to the genus Stutzerimonas, known for species with bioremediation and probiotic properties. To evaluate its genomic features and potential applications, we performed draft-genome sequencing and analysis. The genome of S. kunmingensis TFRC-KFRI-1 was assembled into a 4,756,396 bp sequence with a 62.8% GC content. Genomic analysis suggested potential genes for carbohydrate degradation and lactic acid production. The strain exhibited high average nucleotide identity (ANI) and 16S rRNA similarity with S. kunmingensis HL22-2T, further supporting its potential as a probiotic. This genome sequence provides valuable insights into the functional capabilities of S. kunmingensis TFRC-KFRI-1 and its potential applications in various industries, including aquaculture and food biotechnology. The genome sequence is available under GenBank accession number JBGJJB000000000.1, with related project information under BioProject PRJNA1147901 and Bio-Sample SAMN43173893.
机译:从韩国西海沉积物中的马尼拉蛤蜊肠道中分离出的 Stutzerimonas kunmingensis TFRC-KFRI-1 研究了其作为益生菌的潜力。该菌株属于 假单胞菌科 (Pseudomonadaceae),以前被归类为 Pseudomonas kunmingensis,但后来被重新归类为 Stutzerimonas 属,该属以具有生物修复和益生菌特性的物种而闻名。为了评估其基因组特征和潜在应用,我们进行了草稿基因组测序和分析。将昆明链球菌 TFRC-KFRI-1 的基因组组装成一个 4,756,396 bp 的序列,GC 含量为 62.8%。基因组分析表明碳水化合物降解和乳酸产生的潜在基因。该菌株表现出较高的平均核苷酸同一性 (ANI) 和 16S rRNA 与昆明链球菌 HL22-2T 的相似性,进一步支持其作为益生菌的潜力。该基因组序列为昆明链球菌 TFRC-KFRI-1 的功能能力及其在水产养殖和食品生物技术等各个行业中的潜在应用提供了有价值的见解。基因组序列可在 GenBank 登录号 JBGJJB000000000.1 下获得,相关项目信息可在 BioProject PRJNA1147901 和 Bio-Sample SAMN43173893 下获得。

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