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Primo Vascular System in the Subarachnoid Space of a Mouse Brain

机译:小鼠脑蛛网膜下腔中的Primo血管系统

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摘要

Objective. Recently, a novel circulatory system, the primo vascular system (PVS), was found in the brain ventricles and in the central canal of the spinal cord of a rat. The aim of the current work is to detect the PVS along the transverse sinuses between the cerebrum and the cerebellum of a mouse brain. Materials and Methods. The PVS in the subarachnoid space was analyzed after staining with 4′,6-diamidino-2-phenylindole (DAPI) and phalloidin in order to identify the PVS. With confocal microscopy and polarization microscopy, the primo vessel underneath the sagittal sinus was examined. The primo nodes under the transversal sinuses were observed after peeling off the dura and pia maters of the brain. Results. The primo vessel underneath the superior sagittal sinus was observed and showed linear optical polarization, similarly to the rabbit and the rat cases. The primo nodes were observed under the left and the right transverse sinuses at distances of 3,763 μm and 5,967 μm. The average size was 155 μm × 248 μm. Conclusion. The observation of primo vessels was consistent with previous observations in rabbits and rats, and primo nodes under the transverse sinuses were observed for the first time in this work.
机译:目的。最近,在大鼠的脑室和脊髓中央管中发现了一种新型的循环系统,即原始血管系统(PVS)。当前工作的目的是检测沿小鼠大脑小脑和小脑之间的横窦的PVS。材料和方法。用4',6-二mid基-2-苯基吲哚(DAPI)和鬼笔环肽染色后,分析蛛网膜下腔中的PVS,以鉴定PVS。用共聚焦显微镜和偏振显微镜检查矢状窦下方的primo血管。剥离大脑的硬脑膜和软脑膜后,观察到横窦下方的原始结节。结果。观察到上矢状窦下方的primo血管,并显示出线性光学偏振,类似于兔子和大鼠的情况。在左侧和右侧横窦下方观察到原始结节,其距离为3,763μm和5,967μm。平均尺寸为155μm××248μm。结论。原始血管的观察与先前在兔子和大鼠中观察到的一致,并且在这项工作中首次观察到横窦下方的原始节点。

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