Biomolecule immobilization on nanomaterials is attractive for biosensors since it enables the capture of a higher concentration of bioreceptor units while also serving as a transduction element. The technique could enhance the accuracy, specificity, and sensitivity of the analytical measurements of biomolecules. However, it was found that the limitation in chemically binding biomolecules on nanoparticle surfaces could only cross-link between the C-terminal and N-terminal. Here, we report the facile one-step synthesis of amine-functionalized silica nanoparticles (AFSNPs). (3-Aminopropyl)triethoxysilane was used as a precursor to modify the functional surface of nanoparticles via the Stöber process. The biomolecules were immobilized to the AFSNPs through itaconic acid, a novel cross-linker that binds between the N-terminal and N-terminal and potentially improves proteins and nucleic acid immobilization onto the nanoparticle surface. The newly developed immobilization approach on AFSNPs for biomolecular detection enhanced the efficiency of ELISA, resulting in increased sensitivity. It might also be easily used to identify different pathogens for clinical diagnostics.
展开▼
机译：纳米材料上的生物分子固定化对生物传感器很有吸引力，因为它能够捕获更高浓度的生物受体单元，同时还可以作为转导元件。该技术可以提高生物分子分析测量的准确性、特异性和灵敏度。然而，研究发现，在纳米颗粒表面化学结合生物分子的限制只能在 C 端和 N 端之间交联。在这里，我们报道了胺官能化二氧化硅纳米颗粒 （AFSNP） 的简单一步合成。（3-氨基丙基）三乙氧基硅烷用作前驱体，通过 Stöber 工艺修饰纳米颗粒的功能表面。生物分子通过衣康酸固定在 AFSNP 上，衣康酸是一种新型交联剂，可在 N 端和 N 端之间结合，并可能改善蛋白质和核酸固定在纳米颗粒表面的现象。新开发的用于生物分子检测的 AFSNP 固定化方法提高了 ELISA 的效率，从而提高了灵敏度。它也可以很容易地用于识别不同的病原体以进行临床诊断。
展开▼
