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Activation of the alternative complement pathway by pneumococcal lipoteichoic acid.

机译:肺炎球菌脂蛋白壁酸激活替代补体途径。

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摘要

Cell wall teichoic acids of some gram-positive bacteria are potent activators of the alternative pathway of complement. It is unclear, however, whether the other form of teichoic acid, cell membrane lipoteichoic acid (LTA), can also activate the alternative pathway. In the present study, radiolabelled pneumococcal LTA was found to bind spontaneously to sheep erythrocytes in a temperature- and time-dependent fashion. In addition, the presence of pneumococcal LTA on the erythrocyte surface was verified by the fact that they could be agglutinated by a myeloma protein (TEPC-15) specific for choline, a constituent of pneumococcal LTA. Pneumococcal LTA when fixed to the surface of erythrocytes was able to activate the alternative pathway of complement in both guinea pig serum deficient in the fourth component of complement and human serum deficient in the second component of complement, resulting in lysis of the sensitized erythrocytes. The sensitizing principle of the LTA preparation was removed before erythrocyte sensitization by immunoabsorption, using the choline-specific TEPC-15 myeloma protein. These data demonstrate that purified pneumococcal LTA will bind to sheep erythrocytes and endow them with the ability to activate the alternative pathway.
机译:某些革兰氏阳性细菌的细胞壁软骨酸是补体替代途径的有效激活剂。然而,尚不清楚其他形式的硫磷酸,即细胞膜脂磷壁酸(LTA)是否也可以激活替代途径。在本研究中,发现放射性标记的肺炎球菌LTA以温度和时间依赖性方式自发结合于绵羊红细胞。另外,红细胞表面上存在肺炎球菌LTA的事实是,它们可以被特异性针对胆碱(肺炎球菌LTA的成分)的骨髓瘤蛋白(TEPC-15)凝集的事实所证实。肺炎球菌LTA固定在红细胞表面时,能够激活补体第四成分不足的豚鼠血清和补体第二成分不足的人血清中补体的替代途径,从而导致致敏性红细胞溶解。使用胆碱特异的TEPC-15骨髓瘤蛋白,通过免疫吸收使红细胞致敏之前,无需使用LTA制剂的致敏原理。这些数据表明,纯化的肺炎球菌LTA将与绵羊红细胞结合,并使它们具有激活替代途径的能力。

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