BackgroundThe spore germination rate and growth characteristics were compared between the citric acid high-yield strain Aspergillus niger CGMCC 5751 and A. niger ATCC 1015 in media containing antimycin A or DNP. We inferred that differences in citric acid yield might be due to differences in energy metabolism between these strains. To explore the impact of energy metabolism on citric acid production, the changes in intracellular ATP, NADH and NADH/NAD+ were measured at various fermentation stages. In addition, the effects of antimycin A or DNP on energy metabolism and citric acid production was investigated by CGMCC 5751.
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机译：背景在含有抗霉素A或DNP的培养基中，比较了柠檬酸高产菌株黑曲霉CGMCC 5751和黑曲霉ATCC 1015的孢子萌发率和生长特性。我们推断柠檬酸产量的差异可能是由于这些菌株之间能量代谢的差异。为了探讨能量代谢对柠檬酸生产的影响，在发酵的各个阶段分别测量了细胞内ATP，NADH和NADH / NAD + 的变化。此外，CGMCC 5751还研究了抗霉素A或DNP对能量代谢和柠檬酸产生的影响。
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