首页> 美国卫生研究院文献>International Journal of Fertility Sterility >Comparative Stepwise Pattern of Reactive OxygenSpecies Production during In Vitro Development ofFertilized and Nuclear Transferred Goat Embryos
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Comparative Stepwise Pattern of Reactive OxygenSpecies Production during In Vitro Development ofFertilized and Nuclear Transferred Goat Embryos

机译:活性氧的比较逐步模式体外开发中的物种生产受精和核移植的山羊胚胎

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摘要

BackgroundA unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somatic cell nuclear transfer (SCNT). Furthermore, the pattern of ROS production of SCNT embryos were compared with zona free embryos derived from in vitro fertilization (IVF).
机译:背景技术胚胎代谢的独特特征是产生活性氧(ROS)。公认的是,在体外培养期间,ROS水平在体内发育的胚胎观察到的正常范围内增加。这项研究评估和比较通过体细胞无核移植(SCNT)的无区带方法生产的重建山羊胚胎体外发育过程中ROS产生的逐步模式。此外,将SCNT胚胎的ROS产生模式与源自体外受精(IVF)的无透明带的胚胎进行了比较。

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