Carnosol inhibits inflammasome activation by directly targeting HSP90 to treat inflammasome-mediated diseases

摘要

Western blot analysis of caspase-1 (p20) and IL-1β in culture supernatants (Sup.) and pro- IL-1β, caspase-1 (p45), NLRP3 and ASC in cell lysates (Lys.) of LPS-primed BMDMs treated with various doses of carnosol and then stimulated with nigericin. – Activity of caspase-1 ( ), ELISA of IL-1β ( ), release of LDH ( ) and ELISA of TNF-α ( ) in Sup. from samples described in . Western blot analysis of caspase-1 (p20) and IL-1β in Sup. and pro- IL-1β, caspase-1 (p45), NLRP3 and ASC in cell Lys. of PMA-primed THP1 treated with various doses of carnosol and then stimulated with nigericin. – Activity of caspase-1 ( ), ELISA of IL-1β ( ) and TNF-α ( ) in Sup. from samples described in . , Activity of caspase-1 ( ) and ELISA of IL-1β ( in Sup. from LPS-primed hPBMCs treated with various doses of carnosol and then stimulated with nigericin. Coomassie blue staining was used as the loading control in Sup. GAPDH served as a loading control in the Lys. Data are represented as the mean ± SD from at least four biological samples. The significance of the differences was analyzed using Mann–Whitney test: *  P P