Influenza virus was point-inoculated on to the chick tracheal mucous membrane cultivated on a solid medium (L-15 agar medium) with a platinum microloop (ca 0.03 microliter of inoculum). Following the rapid adsorption of the inoculated virus on the mucosal surface, the explant was infected with a high efficiency. The 50% minimal explant infectious doses (MEID50) of influenza A virus strains (A/PR8/34, A/Kumamoto/79 and A/Bangkok/79) were less than one pfu. Influenza B virus (B/Lee/40) was also adsorbed to the mucous membrane but this virus failed to infect the chick tracheal mucous membrane (MEID50 greater than 10(3.3) pfu). By point-inoculation of virus on to the large tracheal explant (2 X 12 mm), it was possible to trace the behaviour of the infecting virus on the tracheal mucous membrane. Thus, influenza virus A/Kumamoto/79 was shown to be infective at the point-inoculated site by overcoming mucociliary clearance. Thereafter, depending on virus production and the ciliary activity of the infected epithelial cells, the infection spread rapidly to the laryngeal side but more slowly to the bronchial side of the tracheal explant.
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机译:将流感病毒点接种到在带有铂微环(ca 0.03微升接种物)的固体培养基(L-15琼脂培养基)上培养的鸡气管粘膜上。随着接种病毒在粘膜表面的快速吸附,外植体被高效感染。甲型流感病毒株(A / PR8 / 34,A /熊本/ 79和A /曼谷/ 79)的50%最小外植体感染剂量(MEID50)小于1 pfu。乙型流感病毒(B / Lee / 40)也被吸附在粘膜上,但这种病毒未能感染鸡的气管粘膜(MEID50大于10(3.3)pfu)。通过在大型气管外植体(2 X 12毫米)上点病毒接种,可以在气管粘膜上追踪感染病毒的行为。因此,显示出通过克服粘膜纤毛清除作用,流感病毒A /熊本/ 79在点接种部位具有感染性。此后,根据病毒的产生和被感染的上皮细胞的纤毛活性,感染迅速扩散到气管外植体的喉侧,但传播到气管外植体的支气管侧则更慢。
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