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De novo leaf and root transcriptome analysis identified novel genes involved in Steroidal sapogenin biosynthesis in Asparagus racemosus

机译:从头叶和根转录组分析确定了芦笋中甾体皂甙元生物合成的新基因

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摘要

BackgroundSaponins are mainly amphipathic glycosides that posses many biological activities and confer potential health benefits to humans. Inspite of its medicinal attributes most of the triterpenes and enzymes involved in the saponin biosynthesis remains uncharacterized at the molecular level. Since the major steroidal components are present in the roots of A. racemosus our study is focussed on the comparative denovo transcriptome analysis of root versus leaf tissue and identifying some root specific transcripts involved in saponin biosynthesis using high-throughput next generation transcriptome sequencing.
机译:背景皂苷主要是两亲性糖苷,具有许多生物活性,并可能给人类带来健康益处。尽管具有药用特性,但皂苷生物合成中涉及的大多数三萜和酶在分子水平上仍未表征。由于主要的甾体成分存在于沙曲菌的根中,因此我们的研究重点是对根组织与叶组织进行比较性的denovo转录组分析,并使用高通量的下一代转录组测序来鉴定参与皂苷生物合成的某些根特定转录本。

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