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DAMMARENEDIOL SYNTHASE PROMOTER ANALYSIS OF GENES INVOLVED IN SAPONIN BIOSYNTHESIS

机译:皂苷生物合成中所涉及基因的达马烯二酚合成酶启动子分析

摘要

The present invention relates to a promoter of the dammarenediol biosynthetic gene having a sequence of Sequence No. 1, which is a gene involved in the ginseng saponin biosynthesis, and, more specifically, to a sequence of a DDS gene promoter including the 5'-untranslated region of the DDS cDNA(ABI22080) gene, a promoter of dammarenediol biosynthetic gene and a transformation vector including a sequence encoding a target protein operably linked to the promoter. Further, the present invention relates to a transgenic plant in which a vector containing a DDS promoter is introduced into a plant with confirming expression patterns upon time of a DDS promoter in a transgenic mouseear cress and a transgenic ginseng, the influence of hormones and light, and the activity of a DDS promoter to the TMV infection. Accordingly, the promoter is able to be effectively used in developing crops with an increased ginsenoside content, stress-resistant transgenic crops and crops with a reinforced virus resistance, and to be beneficially used in developing transgenic plants for producing medical and industrial proteins.
机译:本发明涉及具有序列号为1的序列的达玛烯二醇生物合成基因的启动子,该序列是人参皂苷生物合成中涉及的基因,更具体地,涉及包括5'-的DDS基因启动子的序列。 DDS cDNA(ABI22080)基因的非翻译区,达玛烯二醇生物合成基因的启动子和包括编码与该启动子可操作连接的靶蛋白的序列的转化载体。此外,本发明涉及一种转基因植物,其中将含有DDS启动子的载体引入转基因鼠耳草和转基因人参中具有确定的DDS启动子时间的表达模式的植物中,激素和光照的影响,以及DDS启动子对TMV感染的活性。因此,该启动子能够有效地用于开发人参皂苷含量增加的作物,抗逆转基因作物和具有增强的病毒抗性的作物,并且可有利地用于开发用于生产医学和工业蛋白质的转基因植物。

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