Molecular cloning characterization genomic organization and promoter analysis of the α16-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0

摘要

BackgroundThe rat hybridoma cell line YB2/0 appears a good candidate for the large-scale production of low fucose recombinant mAbs due to its lower expression of fut8 gene than other commonly used rodent cell lines. However, important variations of the fucose content of recombinant mAbs are observed in production culture conditions. To improve our knowledge on the YB2/0 fucosylation capacity, we have cloned and characterized the rat fut8 gene.