Expression crystallization and preliminary X-ray analysis of the periplasmic stress sensory protein RseB from Escherichia coli

摘要

Sensing external stress in the bacterial periplasm and signal transduction to the cytoplasm are important functions of the CpxAR, Bae and σ^E signalling pathways. In Escherichia coli, the σ^E pathway can be activated through degradation of the antisigma factor RseA by DegS and YaeL. The periplasmic protein RseB plays an important role in this pathway by exerting a direct or indirect negative effect on YaeL cleavage efficiency. RseB from E. coli, missing the periplasmic signal sequence (RseBΔN), was cloned, expressed, purified and crystallized. Crystals were obtained in two different forms belonging to space group P4212 (form I) and C2221 (form II) and diffracted to 2.8 and 2.4 Å resolution, respectively. In crystal form I two copies of the protein were located in the asymmetric unit according to heavy-atom analysis, while crystal form II contained three copies.