Satiety Hormone and MetabolomicResponse to an IntermittentHigh Energy Diet Differs in Rats Consuming Long-Term Diets High inProtein or Prebiotic Fiber

摘要

Large differences in the composition of diet between early development and adulthood can have detrimental effects on obesity risk. We examined the effects of an intermittent high fat/sucrose diet (HFS) on satiety hormone and serum metabolite response in disparate diets. Wistar rat pups were fed control (C), high prebiotic fiber (HF) or high protein (HP) diets (weaning to 16 weeks), HFS diet challenged (6 weeks), and finally reverted to their respective C, HF, or HP diet (4 weeks). At conclusion, measurement of body composition and satiety hormones was accompanied by ¹H NMR metabolic profiles in fasted and postprandial states. Metabolomic profiling predicted dietary source with >90% accuracy. The HF group was characterized by lowest body weight and body fat (P < 0.05) and increased satiety hormone levels (glucagon-like peptide 1 and peptide-YY). Regularized modeling confirmed that the HF diet is associated with higher gut hormone secretion that could reflect the known effects of prebiotics on gut microbiota and their fementative end products, the short chainfatty acids. Rats reared on a HF diet appear to experience fewer adverseeffects from an intermittent high fat diet in adulthood when rematchedto their postnatal diet. Metabolite profiles associated with the dietsprovide a distinct biochemical signature of their effects.