索科线虫是一类具有很大生防潜力的昆虫(如棉铃虫等)天敌资源,但由于其体外培养尚未获得成功,阻碍了商业化生产和大规模应用,究其原因主要是体外培养的线虫不能完成雌雄性别分化.因此,研究该类线虫性别分化机制成为近年本领域的热点课题.该文以中华卵索线虫为实验材料,采用mRNA差异显示的方法,分析了中华卵索线虫性别分化关键时期雌、雄寄生后期幼线虫的基因表达差异.在雌雄线虫体内得到具有表达差异的基因片段20条.其中8条在雄虫体内特异性表达,12条在雌虫体内特异性表达.利用信息生物学技术对所分离到的差异片段进行了序列分析.其中,Ensembl分析发现4个片段与秀丽线虫X染色体具有可匹配片段,推测这些片段可能是影响中华卵索线虫性别分化的重要基因.这些结果将为后续研究提供思路.%Mermithidae, as an important natural predator of pests such as Bollworm, has great potential for natural biological control of invasive pests. Unfortunately, the in vitro culture of the nematode has not yet been successful, delaying the commercial application of this pest control method. The key reason for this failure is the inability of the worms to accomplish sex differentiation, sparking a strong interest in this process. Here, we analyzed the differences in gene expression of female and male postparasitic Ovomermis sinensis juveniles by mRNA differential display. In total, 20 gene fragments that had differential expression in male and female worms were isolated, including 8 male- and 12 female-specific ones. Bioinformatics methods were employed to analyze sequences of these fragments, in which ensembl analysis shows 4 fragments have comparable parts with C. elegence s X chromosome, we speculate those fragments are important genes which influence sex differentiation of Ovomermis sinensis, This data provides an idea for further study of the molecular mechanism of sex differentiation in mermithids.
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