目的：研究Parkin基因甲基化在鼻咽癌早期诊断及预后判断中的价值。方法运用甲基化特异性PCR检测54例鼻咽癌组织、16例慢性鼻咽炎症组织和16例正常鼻咽上皮组织中Parkin基因启动子区甲基化情况，并分析其与鼻咽癌临床生物学因素的关系。结果慢性鼻咽炎症组织和正常鼻咽上皮组织中均未检测到Parkin基因甲基化，而在鼻咽癌组织中甲基化率为62.96%(34/54)，3种鼻咽组织Parkin基因甲基化情况比较有统计学差异（P＜0.05）。进一步统计学分析发现鼻咽癌组织Parkin基因甲基化与其临床生物学因素均无关（均P＞0.05）。结论 Parkin基因甲基化具有肿瘤特异性，与鼻咽癌发生、发展密切相关，有望成为早期分子生物学辅助诊断的标志物，但不能作为判断鼻咽癌预后的预测指标。%Objective To investigate Parkin gene promoter methylation in nasopharyngeal carcinoma (NPC). Methods The methylation- specific PCR (MSP) was used to detect methylation level of Parkin gene in 54 tissue specimens of NPC, 16 specimens of chronic nasopharyngitis and 16 specimens of normal nasopharyngeal epithelia tissues, and its relationship with the clinical and biological features of NPC was analyzed. Results Parkin gene promoter methylation was detected in 62.96%(34/54) of NPC specimens, not in any specimens of chronic nasopharyngitis and normal nasopharyngeal epithelia tissues(P<0.05). Parkin gene methylation was not correlated with clinical and biological variables of NPC (al P>0.05). Conclusion Parkin gene promoter methylation has high specificity in distinguishing NPC from normal nasopharyngeal tissues and inflammatory nasopharyngeal disease, suggesting that it may be used for early molecular diagnosis of NPC.
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