大肠癌不同发展阶段相关基因甲基化定量分析的临床意义

摘要

Objective To investigate the relationship between methylation of relevant genes and the development of colorectal cancer and to assess the value of early diagnosis based on quantitative analysis of methylation of colorectal cancer-related genes. Methods Fluorogenic quantitative polymerase chain reaction (PCR) was applied to detect APC,p16,and colorectal cancer-specific Vimentin gene methylation in colorectal cancer, precancerosis and healthy control groups,respectively. The levels of APC,p16 and Vimentin methylation in different tissues of patients with colorectal cancer were also quantified. Results The methylation rates of the APC,p16 and Vimentin in colorectal cancer group were significant up-regulated compared to those in precancerosis and healthy control groups (P<0.05). Moreover,similar results were also obtained for the levels of APC,p16 and Vimentin methylation in the malignancy,pericarcinomatous and normal tissues in colorectal cancer group (P<0.05). Conclusions The methylation of APC,p16 and Vimentin is involved in the carcinogenesis of colorectal cancer. Quantitative analysis of methylation of colorectal cancer-related genes using fluorogenic quantitative PCR may be used for early diagnosis of colorectal cancer.%目的：通过进行大肠癌患者相关基因甲基化定量分析，探讨相关基因甲基化水平与大肠癌发生、发展的关系。方法采用实时荧光定量PCR技术检测大肠癌组、癌前病变组（结肠腺瘤）及正常对照组的APC、p16及大肠癌特异性基因Vimentin基因甲基化水平。结果大肠癌组中APC、p16及Vimentin基因甲基化阳性率比较癌前病变（结肠腺瘤）和正常肠黏膜组显著升高，其差异有统计学意义（P<0.05）；大肠癌组中肿瘤组织的APC、p16及Vimentin基因甲基化阳性率比癌旁及正常黏膜组织显著升高，其差异有统计学意义（P<0.05）。结论 APC、p16及Vimentin基因甲基化参与了大肠癌的发生发展，且利用实时荧光定量PCR对相关基因甲基化进行定量分析可能预测大肠癌早期发生。