[ Objective ] The purpose of the project was to identify two Morchella ( XJURML4 & XJURML5) collected in Daxigou of Huocheng County, Xinjiang by molecular analysis. [ Method ] The fungi common primer pairs ITS If and ITS4 were applied for amplifying internal transcribed spacer regions of two Morchella, and then RAPD technology were used for sequence analysis. [Result] ITS regions of XJURML4 was 1 130 bp and ITS regions of XJURML 5 was 1 127 bp with EU086775 and EU086776 of GenBank accession numbers respectively. The ITS2 regions are 100% the same and there was only 5bp difference between the regions of the two Morchella. BLAST and Neighbor - joining analysis demonstrated that the homology between the two Morchella and Morchella esculenta (L. Fr. ) Pers. Var. Rotunda Pers. Fr. Were 99. 28% and 98.65% , respectively. Six bands were obtained from each strain by RAPD - PCR; two of them were identical, and the others were conflicting. [Conclusion] ITS regions analysis and RAPD technology demonstrated that XJURML4 and XJURML5 were new varieties in M. Esculenta which was discordant with morphological examination , and also, that the two strains were new variations of Yellow Moles.%[目的]对新疆霍城县大西沟采集的两种形异羊肚菌(XJURML4和XJURML5)进行分类鉴定.[方法]用真菌专一引物ITS1f和ITS4扩增两菌ITS区并进行序列分析.[结果]XJURML4 ITS片段1 130 bp( EU086775),XJURML5 ITS片段1 127 bp(EU086776).两菌的ITS2区100％相似,而ITS1区仅有4 bp差异.BLAST比对测序结果发现XJURML4和XJURML5与近缘种宽圆羊肚菌(MorcheLLa esculenta (L.:Fr.)Pers.var.rotunda Pers.:Fr.)的同源性为99.28％和98.65％；NJ法构建进化树分析ITS序列,两菌也跟宽圆羊肚菌类聚在一起.随机引物OPA3进行RAPD扩增,共产生出六条带,二条相同,剩余四条为多态性条带,表明两菌基因组上也有差异.[结论]ITS区序列和进化树分析、OPA3 - RAPD标记技术均表明两菌是宽圆羊肚菌种内的新变种,而与传统形态学鉴定结果不一致.
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