cqvip:Several studies showed that costimulatory signals on antigen presenting cells are up- regulated in inflammatory bowel disease. We quantified the expression of CD80, CD86, and IFNγ in colonie mucosa of patients affected by ulcerative c olitis and correlated it with clinical and biochemical parameters to identify th e context of this up regulation. We enrolled 21 patients affected by ulcerative colitis and 6 healthy subjects. We evaluated for each patient gender, age, durat ion of disease, clinical, endoscopic and histologic disease activity index, medi cal therapy, ESR, serum CRP, WBC, and serum α 1- acid glycoprotein. CD80, CD86 , and IFNγ expression in the colonie mucosa was quantified using reverse trans cription polymerase chain reaction. Statistical analysiswas performed using Mann - Whitney U test and Spearman’ s rank correlation test. Significance was set a t P<0.05. CD80 was detectable in seven patients, while CD86 and IFNγ expressio n was evident in all UC patients. CD80 and CD86 were not detectable in control s pecimens. Colonie CD80 expression was correlated to the age of the patients. CD8 6 expression showed an inverse correlation with duration of disease and a direct correlation with serum CRP levels and histologic grade of disease activity. IFN γ was not correlated with any of the examined parameter. Our study confirms a major role in ulcerative colitis pathogenesis for CD86 which correlates with his tologic grade of disease and with serum CRP levels, and its up- regulation seem s to be higher at the beginning of the disease. In “ in vivo" conditions IFNγ may not be the only factor responsible for CD86 up- regulation in the ulcerati ve colitis colonic mucosa.
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