食管癌术后切缘复发的分子生物学研究

摘要

Objective to inquire into the molecular biological causes for effecting relapse of esophageal carcinoma on the resected edge, we observe the express circumstances of Pcna, cyclind1, cea, e-cadherin and P53 on the resected edge of esophageal squamous cell carcinoma after operation with following comparative research. then it can provide theory basis for calculation of high and dangerous recurrent crowd and guiding clinical treatment. Methods the immunohistochemical sP staining method of expression of above five genes was respectively per-formed on the resected edge and main body lump of both contrary group and relapse group. analyze the expres-sional relation between the P53 and e-cadherin protein. Results(1)the positive expression rate of P53 and e-cad-herin protein showed on the resected edge of relapse group was significantly different with that of comparative group.(2) the positive expression rate of P53、e-cadherin and cea protein on the main body lumps of relapse group was significantly different with that of comparative group. (3)the expression of P53 had negative relation-ship with that of e-cadherin significantly.Conclusions Various genes possibly participate in the cutting margin relapse of esophageal carcinomas after operation. the higher P53 expression and the lower e-cadherin expression on the cutting edge is possible molecular basis of relapse, and also the two genes probably involve themselves in the process of relapse together. so the expression of P53 and e-cadherin can be used as two important biological indicators for calculating resected edge recurrence of esophageal carcinomas.%　　目的通过观察 Pcna、cyclind1、cea、P53、e-cadherin 5种基因在食管鳞状细胞癌术后切缘的表达情况及对比研究，探讨影响食管癌术后切缘复发的分子生物学因素，为预测术后复发的高危人群及指导临床治疗提供理论依据。方法应用免疫组织化学方法对对切组(对照组切缘)和复切组(复发组切缘)及复主组(复发组主体癌)和对主组(对照组主体癌)进行上述5种基因表达水平的检测。结果(1)P53和 e-cadherin 表达阳性率在复切组和对切组间差异均具有显著性意义。(2)P53、e-cadherin 和 cea 蛋白表达阳性率在复主组与对主组间差异均具有显著性意义。(3)P53与 e-cadherin 的表达具有显著负相关性。结论食管癌术后切缘复发可能由多基因参与。切缘 P53高表达、e-cadherin 低表达可能是食管癌术后切缘复发的分子基础，且两者可能共同参与切缘复发。因此 P53、e-cadherin 可作为预测食管癌切缘复发的重要生物学标志。