头穴丛刺对大鼠缺血脑组织血管内皮生长因子表达的影响

摘要

目的:探讨头穴丛刺法对大鼠缺血脑组织血管内皮生长因子(VEGF)表达的影响.方法:128只大鼠随机分为假手术组、模型组、头针组和头穴丛刺组;头针组大鼠取病灶侧百会透曲鬓,头穴丛刺组大鼠双侧百会透曲鬓及百会穴;均进针2mm,持续捻转10min,留针2h.以免疫组化法测定血管内皮生长因子的表达,测定微血管密度.结果:在缺血后各时间点,经头穴丛刺干预后的VEGF表达明显增强,优于头针组;在7d、14d、21d三个时间点,经头穴丛刺干预后微血管密度增多,优于头针组.结论:头穴丛刺法可以通过促使VEGF表达上调,促进微血管新生.%objective To probe into the impacts on the expression of vascular endothelial growth factor(VEGF)in ischemic brain tissue treated with cluster puncture on scalp acupoints in rats.Methods 128 rats were randomized into pseudo-operation group,model group,scalp-needling group and cluster-needling group.In scalp-needling group,penetration method was used on the focal side from Bǎihui(百会 GV20)to Qǔbūn(曲鬓 GB7),and in cluster-needling group,penetration method was used on both sides from GV20 to GB7,and a common needling on GV 20.Needles were punctured 2mm in depth,constantly rotated for 10min,retained for 2h.Immunohistrochemical method was applied to determine VEGF expression and microvessel density(MVD).Results With the intervention of cluster puncture on scalp acupoints,VEGF expressions on every time-spot after ischemia were enhanced apparently,superior to those in scalp-needling group.On the three time-spots of the 7th,14th and 21st days,MVD was increased after cluster puncture on scale acupoints,superior to those in scalp-needling group.Conclusion Cluster puncture on scalp acupoints up-regulated VEGF expression and promoted regeneration of microvessel.