维生素K3增强三氧化二砷诱导多发性骨髓瘤细胞凋亡的实验研究

摘要

目的:探讨维生素K3对三氧化二砷(As2O3)诱导人多发性骨髓瘤(MM)细胞株RPMI.8226细胞凋亡的作用及生长抑制.方法:采用四甲基噻唑氮蓝比色法检测As2O3联合维生素K3对人多发性骨髓瘤(MM)细胞株RPMI.8226细胞生长抑制作用;流式细胞术检测As2O3联合维生素K3对RPMI8226细胞周期和凋亡率的影响.结果:单用As2O3对RPMI.8226细胞的生长有抑制作用,其作用呈时间和剂量依赖性;与单用As2O3相比,As2O3联合维生素K3作用于RPMI.8226细胞48h后,细胞凋亡率显著增加(P<0.05),G0/G1期细胞比例升高,S期减低,并有凋亡峰.结论:维生素K3可以增强As2O3对RPMI8226细胞诱导凋亡作用.%Objective:To investigate the effect of VK, on arsenic trioxide induced apoptosis of multiple myeloma RPMI8226 eells and the molecular mechanisms. Methods:Growth inhibition of RPMI8226 cells exposed to Vitamin K3 Combination with As2O3 was analyzed by MTT assay,Anncxin V labeling. Results:As203 alone could inhibit the growth and induce apoptosis of RPMI 8226 cells,whil e As2O3 inhibited the proliferation of RPMI8226 cells in a time and dose - dependent manner. Compared with As2O3 treatment alone,growth inhibition was enhanced by combination therapy with As2 O3 and VK, (P<0.05), Annexin V staining showed the earlier apoptotie cells. Flow eytometry showed that the proportion of G0/G1 cells was increased and cell proportion in phase was decreased with appearance of apoptosis peak. Conclusion: VK3 enhances the apoptosis induced by arsenic trioxide in RPMI8226 cells.