风药、补虚药对脑缺血大鼠淀粉样β蛋白及其蛋白前体表达的影响

摘要

Objective:To observe the effects of wind-dispelling medicine, deficiency-nourishing medicine, wind-dispelling medicine as-sisted with deficiency-nourishing medicine of Houshiheisan on the expression of APP and Aβ42 in cerebral ischemic rats.Methods:Lo-cal cerebral ischemia injured rats was replicated by occlusion of the right middle cerebral artery .The immunohistochemistry and MIPS were used to measure the expressions of APP and Aβ42 in the cortex after cerebral ischemia.Results: Expressions of APP and Aβ42 were significantly increased in model group in comparison with sham group (P<0.01).Twenty-four hours after cerebral ischemic oc-curred, deficiency-nourishing medicine and combination group reduced expression of APP .There was a significant difference in compari-son with model group (P<0.05).Forty-eight hours after cerebral ischemic occurred , wind-dispelling medicine reduced expression of APP.There was a statistically difference in comparison with model group (P<0.05).Forty-eight hours after cerebral ischemic oc-curred, combination group could reduce expressions of Aβ42.There was a significant difference in comparison with the model group (P<0.01).Conclusion:Wind-dispelling medicine assisted with deficiency-nourishing medicine of Houshiheisan can reduce the expres-sion of APP, inhibition aggregation of Aβ42, and the cytoxicity of Aβ42, thus resisting the ischemia injure.%目的：观察侯氏黑散方中风药、补虚药、风药补虚药联用对脑缺血大鼠淀粉样前体蛋白（amyloid precursor protein，APP）、β-淀粉样蛋白（Aβ）表达的影响。方法：线栓法阻断大鼠大脑中动脉，制备局灶性脑缺血大鼠模型，采用免疫组化与医学图像分析相结合的方法检测侯氏黑散方中风药、补虚药、风药补虚药联用对APP及Aβ42表达的影响。结果：脑缺血24 h、48 h，模型组大鼠皮层APP、Aβ42表达较假手术组明显增强（P＜0.01）。缺血24 h，补虚药、风药补虚药联用可明显下调APP表达，与模型组比较差异有统计学意义（P＜0.05）；缺血48 h，风药组大鼠皮层APP表达较模型组明显降低（P＜0.05）；缺血48 h，风药补虚药联用可明显降低Aβ42表达，与模型组相比有统计学意义（P＜0.01）。结论：风药补虚药联用可明显下调APP表达，抑制Aβ42的异常积聚，降低Aβ42的毒性，从而发挥抗脑缺血损伤的作用。