抗氧化成分白藜芦醇的树脂分离工艺研究

摘要

目的：研究大孔吸附树脂分离纯化虎杖中白藜芦醇的工艺条件及参数。方法：利用白藜芦醇显酸性的特点，采用碱液洗脱树脂法分离纯化白藜芦醇。考察6种树脂、碱的浓度以及洗脱流速等因素对白藜芦醇分离纯化的影响。结果：选择NKA－Ⅱ树脂，NaOH洗脱液浓度0．5％，流速为1 BV/h，经过去盐后，白藜芦醇纯度达到70％以上。结论：该工艺适用于对白藜芦醇有吸附的树脂，经过一次上柱就可以与原料中其他干扰成分分离，适合大规模生产。%Objective:To explore purification techniques of Resveratrol by macroporous adsorption resin.Methods:As resveratrol is a-cidic, lye elution resin separation and purification was used.The impact of resin types, NaOH concentration, flow rate on purification results were explored.Results:The optimized purification techniques was: NKA-Ⅱ resin, eluent concentration of NaOH 0.5%, flow rate=1BV/h, after desalting, resveratrol purity >=70%.Conclusion:The optimized purification techniques is applicable to adsorp-tion resin that is resveratrol adsopting, separation with other ingredients can be achieved by one flow, it is suitable for mass production.