左右利手捻转补泻手法对血虚证模型大鼠 RBC、Hb、IRF 的影响

摘要

Objective:To explore the relations between the twisting-twirling reinforcing-reducing manipulation of acupuncture and the twisting-twirling direction.Methods:Eighty Wistar rats were randomly divided into 7 groups, including a blank group (group A), model group (group B), acupuncture group (group C), right-hand-forward twisting group (group D), right-hand-backward twisting group (group E), left-hand-forward twisting group (group F) and left-hand-backward twisting group (group G).All the rats were made into blood-deficiency models by tail-bleeding and food restriction .Acupuncture was given every other day with 10 sessions totally, and the levels of red blood cell (RBC), hemoglobin (Hb), immature reticulocyte fraction(IRF) were measured at different time periods.Re-sults:One day before the treatment, the RBC and Hb of all the groups, except the blank group (A group), decreased significantly (P<0.01), while the IRF increased obviously (P<0.01).On the 7th day of the treatment, the RBC and Hb of each group were measured and compared respectively, and the results were as follows:(a) group B was the lowest (P<0.05);(b) no statistical differences (P>0.05) were found among the group C, group D and group F;(c) no statistical differences (P>0.05) were observed among the group A, group E and group G;( d) The RBC and Hb of the first three groups were obviously higher than those of the latter three groups ( P<0.05).Besides no significant IRF differences (P>0.05) were found among all the treatment groups .No statistical differences (P>0.05) were observed among all the groups on the 14th and 21th day of treatments.Conclusion:There exists no essential relation between the twisting-twirling reinforcing-reducing manipulation and the right or left twisting-twirling direction.Moreover, thumb forward twisting is “reinforcing method”, while thumb backward twisting is “reducing method”, no matter using the right or the left hand .%目的：探讨捻转补泻手法与具体捻转方向的关系。方法：将80只Wistar大鼠随机分为7组：空白组（ A组）、模型组（ B组）、留针组（C组）、右手前捻组（D组）、右手后捻组（E组）、左手前捻组（F组）、左手后捻组（G组）。采用放血加限食法制备血虚证模型。隔日针刺1次，共10次。检测大鼠红细胞计数（RBC）、血红蛋白（Hb）、幼稚网织红细胞比率（IRF）。结果：治疗前1 d，与A组相比，其余各组RBC、Hb显著降低（P＜0.01），IRF显著升高（P＜0.01）；治疗第7 d，各组RBC、Hb比较，B组含量最低（P＜0.05），C、D、F 3组差异不明显（P＞0.05），A、E、G 3组差异无统计学意义（P＞0.05），但前3组含量高于后3组（P＜0.05），IRF组间差异不明显（P＞0.05）；治疗第14、21 d，各治疗组RBC、Hb、IRF组间差异均不明显（P＞0.05）。结论：捻转补泻手法与左、右捻转方向无关，无论何手持针，均拇指前捻用力为“补”，拇指后捻用力为“泻”。