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首页> 外文期刊>BMC Complementary and Alternative Medicine >Methanol (80%) leaf extract of Otostegia integrifolia Benth (Lamiaceae) lowers blood pressure in rats through interference with calcium conductance
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Methanol (80%) leaf extract of Otostegia integrifolia Benth (Lamiaceae) lowers blood pressure in rats through interference with calcium conductance

机译:左右甲醇(80%)左右左右左右左右左右左右叶片(LamiCeae)通过干扰导电的干扰降低了大鼠的血压

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摘要

Otostegia integrifolia Benth. (Lamiaceae) leaves are used to treat hypertension in Ethiopian folk medicine. However, the claim has so far not been investigated scientifically. Thus, the objective of this study was to evaluate the antihypertensive activity of 80% methanol leaf extract of O. integrifolia in animal model of hypertension and possible underlying mechanisms in isolated rat aorta. Antihypertensive effect of various oral doses of the extract (250, 500 and 1000?mg/kg) was determined in fructose-induced hypertensive rats using the non-invasive tail-cuff method. Thoracic aortic strips of rats were isolated and suspended in organ bath, and the vasodepressor effect as well as the possible mechanism (s) of action were studied by means of isometric tension recording experiments ex vivo. Phytochemical analysis was also performed to suggest possible constituents related to the activity. Blood pressure was significantly lowered in a dose-dependent manner following extract administration, suggesting that the extract possesses antihypertensive activity. The extract also caused a dose-dependent relaxation of aortic strip precontracted with KCl at a concentration of 6.25–125?μg/L, with a maximum relaxation (100%) achieved at a cumulative concentration of 318.75?μg/ml. The relaxation mechanism was found to be independent of muscarinic receptors, prostanoids, histamine receptors, ATP dependent K channels, sarcoplasmic reticulum stored Ca2 and the endothelium system. The extract shifted the Ca2 concentration-response curve to the right similar to that caused by nifedipine, suggesting that vasorelaxation could possibly be mediated via calcium channel blockade. The extract was found to contain phenolic compounds (164.3?mg/g, expressed as gallic acid equivalents) and flavonoids (125.7?mg/g, expressed as quercetin equivalents). The findings revealed that the plant is endowed with antihypertensive activity, providing evidence for its traditional use. The effect maybe, at least in part, due to dilation of blood vessels through blockade of Ca ?2 channels mediated by phenolic and flavonoid constituents.
机译:ostostegia entegrifolia benth。 (Lamiaceae)叶用于治疗埃塞俄比亚民间医学的高血压。但是,索赔迄今未经科学研究。因此,本研究的目的是评估O. Integifolia的80%甲醇叶提取物的抗高血压活性在高血压动物模型中的胃模型和孤立的大鼠主动脉中可能的潜在机制。使用非侵入性尾巴套管法测定各种口服剂量的抗高血压效果萃取剂(250,500和1000μl)在果糖诱导的高血压大鼠中测定。分离胸部主动脉丝,并悬浮在器官浴中,蒸发器效应以及通过等距张力记录实验进行的辐射记录实验研究了可能的作用机制。还进行了植物化学分析,以表明可能与活动有关的组分。在提取物给药后以剂量依赖性方式显着降低血压,表明提取物具有抗高血压活性。提取物还引起了用KCl的浓度为6.25-125Ωμg/ L的主动脉带的剂量依赖性弛豫,以6.25-125Ωμg/ L的浓度,最大弛豫(100%)以累积浓度为318.75Ω·μg/ ml。发现弛豫机制与毒蕈碱受体,前列腺素,组胺受体,ATP依赖性K通道,肌肉网储存CA2和内皮系统无关。提取物将Ca2浓度 - 响应曲线移位到类似于硝苯地平引起的右侧的右侧,表明血管内膨胀可能通过钙通道封闭来介导。发现提取物含有酚类化合物(164.3×mg / g,表示为无碱酸当量)和黄酮类化合物(125.7×mg / g,表达为槲皮素等同物)。结果表明,该植物赋予抗高血压活动,为其传统使用提供了证据。由于通过酚类和黄酮组分介导的CAα2通道阻断血管扩张,效果可能是至少部分原因。

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