基于“温补脾阳”功效的附子“效-毒-证”关联研究

摘要

Objective:To investigate efficacy and toxicity of Fuzi water extract for control and treatment of yang deficiency of spleen and kidney in rats .Methods:Establish rat model of syndrome of yang deficiency of spleen and kidney by administering 15%rhubarb powder suspension to rat for 9 days, 8.9g· kg -1 · d-1 everyday and then making it swim in cold water until stressed out .The prevention group was establishing the syndrome model and simultaneously treated with Fuzi .The treatment group was treated after the disease model was established successfully .Treatment duration was 9 days.Syndrome scores and weight were collected , and determined content level of TSH, IgG and cardiac toxicity index C 3, LDH, CTn-I.Results:Fuzi water extract could improve the rat model of yang deficiency of spleen and kidney , increase spleen weight , kidney weight , adrenal gland weight and thymus weight to body weight , increase the level of serum TSH, IgG, C3 and LDH, CTn-I, and decrease heart to body weight ratio .Conclusion:Fuzi water extract has preventing and cu-ring effect on syndrome of yang deficiency of spleen and kidney and has certain cardiac toxicity .%目的：分别研究附子水提物预防或治疗给药对脾肾阳虚证大鼠发挥温补脾阳功效时的药效及其伴随毒副作用。方法：按15％大黄粉悬液每天给药剂量为8.9 g· kg-1· d-1，连续给药9 d，且附加冰水游泳致力竭的方法，制备大鼠脾肾阳虚证模型。预防实验组给药与造模同时进行，治疗实验组造模成功后给药，两个实验药物组均给药9d。实验期间进行证候积分和体重称量，并检测血中TSH、IgG、C3等药效指标和LDH、CTn-I等心毒性指标的水平。结果：附子水提物能改善脾肾阳虚证大鼠的证候积分，增大脾体比值、肾体比值、肾上腺体比值和胸腺体比值，同时能升高血清中TSH、IgG、C3水平，且升高血LDH、CTn-I的水平，降低心体比值。结论：附子水提物对大黄致脾肾阳虚证大鼠具有一定的预防和治疗作用，同时均伴有一定的心脏毒性作用。