消石利尿化瘀法降低高尿酸血症大鼠血清尿酸及其机理的研究

摘要

目的：研究以消石利尿化瘀法为指导创制的中药复方对高血酸血症大鼠血清尿酸的影响，并对其作用机理进行探讨。方法：雄性 Wistar 大鼠随机分为空白组、模型组、痛风定胶囊组、苯溴马隆组及中药复方组（6.07 g／kg、3.03 g／kg）共6组。除空白组外，其余各组以腺嘌呤和乙胺丁醇灌胃，同时各给药组灌胃给予相应的药物；空白组和模型组给予等体积蒸馏水，连续10 d。末次给药后，取血，分离血清，以紫外分光光度法测定大鼠血清尿酸（UA）、尿素氮（BUN）的含量及黄嘌呤氧化酶（XOD）的活性；用放射免疫分析法测定大鼠血清中尿酸盐转运蛋白（UAT）、有机阴离子转运蛋白（OAT）的含量。结果：中药复方高、中剂量组大鼠血清 UA 含量较模型组均降低（P ＜0.05，P ＜0.01）；中药复方高剂量组大鼠血清 XOD 活性与模型组比较，明显降低（P ＜0.01）；中药复方中剂量组大鼠血清 BUN 含量较模型组显著降低（P ＜0.05）；中药复方高剂量组大鼠血清 UAT 含量与模型组比较，明显升高（P ＜0.01）；中药复方高、中剂量组大鼠血清 OAT 含量与模型组比较，均明显升高（P ＜0.01）。结论：以消石利尿化瘀法创制的中药复方能降低高尿酸血症大鼠血清尿酸含量；其降尿酸机理可能与抑制 XOD 活性、减少尿酸生成，以及保护肾功能、增加 UAT、OAT 的含量、促进尿酸分泌与排泄有关。%Objective:To investigate the effect and mechanism of Chinese herbal compounds prescription,which based on the tra-ditional therapeutics of “expelling stone,diuresis and removing stasis”,on uric acid (UA)in rats with hyperuricemia.Methods:Male Wistar rats were randomly divided into normal group,model group,Tongfengding Capsules group,Benzbromarone group and Chinese herbal compounds prescription group(TFK)in two doses of 6.07 g·kg -1 and 3.03 g·kg -1 .In addition to the normal group,the rest were offered with Ademine and the Ethambutol for 10 days.While the appropriate drugs administered to the rats of the administration group,the normal group and the model group were given by gavage distilled water for 10 days.Then blood was collected,UA,BUN content and XOD activity in serum were determined by ultraviolet visible spectroscopy while UAT、OAT content in serum were detected by radiommunoassay.Results:Compared with the model group,the concentration of UA in high-dose and middle-dose Chinese herbal compounds prescription group significantly decreased in serum(P <0.05,P <0.01).The level of XOD activity of high-dose group significantly decreased as compared to the model group (P <0.01).The BUN content of middle-dose group was decreased in serum compared to the model group (P <0.05).The UAT content of high-dose group com-pared to the model group was significantly increased (P <0.01).The content of OAT in high-dose and middle-dose group was sig-nificantly increased in serum compared to the model group (P <0.01).Conclusion:The Chinese herbal compounds prescription which based on the traditional therapeutics of “expelling stone,diuresis and remove stasis”decreased the uric acid content in hy-peruricemia model rats.The mechanism may be related to the relieving effect of the activation of XOD,reducing generation of uric acid,protecting renal function,increasing of UAT and OAT content and promoting excretion of uric acid.