气温骤升骤降对金黄地鼠血清MT、IL-1、IL-6及T淋巴细胞的影响

摘要

目的：部分揭示气候突变对正常机体免疫功能的影响，为进一步研究极端气候变化对人体健康的影响奠定基础。方法：选用人工模拟系统，进行气温骤升、骤降干预。在干预24 h、72 h 后取血，测定金黄地鼠血清 MT、IL-1、IL-6及 T 淋巴细胞免疫相关指标。结果：1）与正常气候组比较，在气温骤升干预24 h 后，血清 IL-1和 IL-6含量明显升高，其差异有统计学意义（P ＜0．01）。72 h 时，血清 MT、IL-1、IL-6水平下降，接近正常对照组含量；气温骤降干预后24 h，IL-1升高明显，差异有统计学意义。72 h 后，血清 MT、IL-1和 IL-6水平均表现为明显升高，差异有统计学意义。2）在气温骤升24 h 时，和正常气候组比较，CD3＋、CD4＋水平升高，有统计学意义（P ＜0．05）。CD8＋表现为降低，其差异无统计学意义（P ＞0．05）。72 h 后，CD3＋和 CD4＋恢复至正常水平，而 CD8＋则进一步降低，差异有统计学意义（P ＜0．01）；气温骤降干预时，CD3＋水平升高，72 h 后差异有统计学意义（P ＜0．05）。CD4＋未出现明显变化，CD8＋在干预24 h 后明显降低（P ＜0．05），72 h 时基本恢复至正常水平；在气温骤升和骤降时，CD4＋／CD8＋均表现为升高趋势，差异有统计学意义（P ＜0．05）。3）气温骤升结束24 h 时，MT 与 IL-6、CD3＋、CD8＋均显著正相关，相关系数分别为0．779、0．836、0．828；气温骤升结束72 h 时，MT 与CD3＋、CD4＋、CD8＋均显著正相关，相关系数分别为0．923、092、0．883、0．792；气温骤降结束24 h 时，MT 与 CD3＋、CD4＋、CD8＋均显著正相关，相关系数分别为0．86、0．818、0．807、0．783；气温骤降结束72 h 时，MT 与 IL-1、IL-6、CD3＋、CD4＋、CD8＋正相关，结论：血清 MT、IL-1、IL-6、CD4＋／CD8＋在气温突变时水平升高，可能为气候突变影响机体免疫力的主要机制之一。MT 与 CD3＋、CD4＋、CD8＋存在显著正相关，分析其机制，可能在气温骤变条件下，机体分泌 MT 的能力增强，血清 MT 含量增加，加快了 T 细胞的增殖速度，从而提高机体免疫力，以此对抗气温骤变带来的不利影响。%Objective:To illustrate the effect of sudden increase and drop in temperature on immunity in normal conditions,and to lay the foundation of a further study on effect of extreme climate change on human bodies.Methods:Manual simulation system was adopted to simulate sudden increase and drop in temperature.Blood tests were applied for measuring serum MT,IL-1,IL-6 and T-lymphocytes of golden hamsters after 24 hours and 72 hours of intervention respectively.Results:1)Compared with the normal cli-mate group,IL-1and IL-6 levels in serum increased significantly during the 24 hours after a sharp increase in temperature (P <0.01).MT,IL-1 and IL-6 levels in serum decreased,almost the same as the normal control group content after a 72 h sharp in-crease in temperature;IL-1 levels increased greatly (P <0.01)after a 24 h sudden drop in temperature.MT,IL-1 and IL-6 lev-els in serum were significantly increased after a 72 h sudden drop in temperature.All data above showed statistical significance.2) Compared with the normal climate group,CD3 + and CD4 + levels significantly increased(P <0.05)after 24 hours of sharp in-crease in temperature.CD8 + level decreased,but the difference was not statistically significant(P >0.05).After 72 h,CD3 +and CD4 + levels were normal again,but CD8 + level continued to reduce,and the difference was statistically significant.CD3 +level increased and there was a significant difference after 72 h.CD4 + Contents had no significant change.CD8 + level significant-ly decreased after 24 h of intervention(P <0.05)and recovered to normal level when 72 h.CD4 +/CD8 + level tended to increase in sudden change of temperature(P <0.05)with statistical significance.3)After 24 h increase of temperature,MT and IL-6, CD3 + and CD8 + had positive correlation,and correlation indexes were 0.779,0.836,0.828;after 72 h increase of temperature, MT and CD3 +,CD4 + and CD8 + had positive correlation,and correlation indexes were 0.923,092,0.883,0.792;after 24 h de-crease of temperature,MT and CD3 +,CD4 + and CD8 + had positive correlation,and correlation indexes were 0.86,0.818, 0.807,0.783;after 72 h decrease of temperature,MT and IL-1,IL-6,CD3 +,CD4 + and CD8 + had positive correlation.Con-clusion:Serum MT,IL-1,IL-6 and CD4 +/CD8 + levels all increased significantly in sudden change of temperature,which may be one of the main mechanisms of effect of abrupt climate changes on immunity.MT and CD3 +,CD4 + and CD8 + had significantly positive correlation,which may because of more secretion of MT,increased content of MT in serum,and the multiplication rate of T-lymphocytes improved,thus the immunity was developed to cope with sudden change of temperature.