羊耳菊水提物对肺炎克雷伯菌所致重症肺炎大鼠的抗炎效果及机制

摘要

目的:探讨羊耳菊水提物对肺炎克雷伯菌所致重症肺炎大鼠的抗炎效果及机制.方法:采用肺炎克雷伯茵诱导大鼠重症肺炎模型,羊耳菊水提物(6,12和24 g/kg体重)灌胃治疗,地塞米松(1.04 mg/kg体重)为阳性对照,6d后测定支气管肺泡灌洗液中白细胞和中性粒细胞以及肺组织TNF-α,IL-1β,IL-6水平及MPO的活性,Western-blot法检测肺组织NF-κBp65、p-p38MAPK蛋白水平.结果:不同剂量羊耳水提物能改善动脉血气指标,减少白细胞及中性粒细胞的水平,降低肺组织TNF-α、IL-1β、IL-6、MPO水平及NF-κBp65、p-p38MAPK蛋白水平.结论:羊耳菊水提物能通过阻断p38MAPK和NF-κBp65信号通路改善肺炎克雷伯菌所致大鼠重症肺炎的炎性反应,可成为一种天然有效的抗炎药物.%Objective:To observe anti-inflammatory effect of Inula cappa aqueous extract on Klebsiella pneumoniae-induced severe pneumonia in rats.Methods:Klebsiella pneumoniae was used to induce ultrasonic severe pneumonia model in rats.Different doses of Inula Cappa aqueous extract (6,12 and 24 g/kg BW) were orally given,and take dexamethasone (1.04 mg/kg weight) as the comparison drug to conduct blood gas analysis,including peripheral white blood cells,neutrophils,TNF-alpha,IL-1 beta,IL-6,LDH and MPO activity in lung tissue.Results:Different doses of Inula Cappa aqueous extract can improve the arterial blood gas index,reduce the content of white blood cells and neutrophils,decrease the content of TNF-α,IL-1 β,IL-6,MPO and level of NF-KBp65 and p-p38MAPK.Conclusion:Inula Cappa aqueous extract can improve inflammation of severe pneumonia induced by Klebsiella pneumoniae by blocking p38 MAPK and NF-KBp65 signaling pathways.It can be taken as a natural and effective anti-inflammatory drug.