寄生软化汤诱导骨肉瘤小鼠的免疫效应

摘要

目的:探讨寄生软化汤对骨肉瘤小鼠免疫功能的诱导作用,为肿瘤的中药辅助治疗提供参考.方法:选取昆明种小鼠,随机分为模型给药组、模型组、空白模型组、正常组,其中模型给药组腹腔注射给予25 mg/kg顺铂及给予13 mg／kg寄生软化汤灌胃,模型组给予相同剂量的顺铂和生理盐水,空白模型组和正常组分别给予相同剂量的生理盐水.疗程结束后统计分析小鼠体重、肿块近似体积、脏器指数及T细胞亚群.结果:与模型组比较,寄生软化汤可明显提高骨肉瘤小鼠的体重及CD3 +T细胞、CD4 +T细胞、CD8 +T细胞百分率及CD4 +／CD8 +比值(P＜0.05).运用寄生软化汤的小鼠较模型组肿块体积减少,胸腺和脾脏指数增加,但变化并不显著.结论:寄生软化汤对骨肉瘤小鼠的免疫功能具有调节和诱导作用.中药与化疗联合治疗肿瘤可增强机体免疫功能,抑制肿瘤生长.%Objective:To investigate the immune inducing activity of Jisheng Ruanhua Decoction on mice with osteosarcoma and to provide references for synergetic treatment of tumor with traditional Chinese Medicine .Methods:Kunming mice were randomly di－vided into model administration group , model group, blank model group and normal group.The model administration group re－ceived intraperitoneal injection of 25mg/kg cisplatin and intragastric administration of 13mg/kg Jisheng Ruanhua Decoction , and the model group was administrated the same dosages of cisplatin and saline with the blank model group and the normal group being given the same dosage of saline respectively.After the end of the treatment course , the body weight, approximate volume of tumor , organs index and T cell subgroups were statistically analyzed.Results:Compared with the model group , Jisheng Ruanhua Decoction could significantly increase the body weight and the percentage of CD 3 +T cell, CD4 +T cell, CD8 +T cell and CD4 +/CD8 +ratio of the mice with osteosarcoma (P＜0.05).Furthmore, Jisheng Ruanhua Decoction reduced the volume of the tumor and improved the index of thymus or spleen with no remarkable changes.Conclusion:Jisheng Ruanhua Decoction could regulate and induce im－mune function of mice with osteosarcoma.The combination treatment of chemotherapy and traditional Chinese medicine enhanced the immune function and inhibited tumor growth.