基于'肾应冬'理论探讨季节变化对金黄地鼠免疫系统的影响

摘要

目的:观察松果体摘除术金黄地鼠白介素-1(Interleukin-1,IL-1)、IL-2、IL-6及IgE、IgG在四季中的水平并与正常金黄地鼠进行比较,探讨松果体对免疫系统的季节性调控机制,揭示"肾应冬"的内涵.方法:选取2个月龄金黄地鼠,分为春分、夏至、秋分、冬至4个节气组,每个节气组内又分实验组(行松果体摘除术)、对照组.动物饲养至春分、夏至、秋分、冬至4个时间点当日,于晚9:00后腹主动脉取血,用酶联免疫吸附试验测定相关免疫指标.结果:IL-2、IL-6、IgE、IgG的水平变化具有明显的季节节律性;IL-1、IL-2、IL-6、IgG水平在冬季达到最低;实验组和对照组间比较,显示松果体能调控IL-2、IgE、IgG的分泌,IL-2在被摘除松果体后失去了季节变化的规律性.结论:血清白介素和免疫球蛋白水平基本存在春夏高、秋冬低的季节性变化规律,这与中医"冬季封藏"的理论不谋而合,且松果体在此过程中对免疫系统发挥部分调节作用.%Objective:To discuss the effect of the pineal in modulating the immune system and the connotation of"kidney corre-sponds with winter"through observing the level of golden hamster's interleukin-1(IL-1),IL-2,IL-6 and IgE and IgG in seasons after removing the pineal and comparing it with normal golden hamsters.Methods:Two-month-old Golden hamsters were divided into four groups:vernal equinox,summer solstice,autumn equinox and winter solstice.Each group was subdivided into experimental group(row pineal extirpation)and control group.Golden hamsters were fed until vernal equinox,summer solstice,autumnal equi-nox and winter solstice.Blood was drawn from abdominal aorta on these four days after 9:00 pm,and ELISA method was used to measure the relevant immune index.Results:The content of IL-2,IL-6,IgE and IgG had obvious seasonal rhythmicity.The content of IL-1,IL-2,IL-6 and IgG reached the lowest in winter.It was shown that the pineal could regulate the secretion of IL-2,IgE and IgG,and IL-2 lost the regularity of seasonal change after removing the pineal.Conclusion:The content of serum interleukin and im-mune globulin basically is high in spring and summer and low in autumn and winter,which coincides with the theory of"winter-sto-ring"in traditional Chinese medicine.And the pineal can partly regulate the immune system.