In order to establish a high frequency system of tissue culture and plant regeneration in Fraxinus rhynchophylla, taking adventitious stem segments with buds induced from dormant bud and seedling in F. Rhynchophylla as explants, the optimal nutrient media and different combinations of plant growth regulators were selected out, multiple shoots were induced and regenerated plants were obtained. The results showed that April was superior to other periods for collecting dormant buds. The proliferation coefficient of shoots from dormant bud was 2.32 in improved B5 medium supplemented with 4 mg · L-1 BA and 0.10 mg · L-1 IBA, and the proliferation coefficient of shoots from aseptic seedling in the same medium was over 3.0, so the medium was optimal. Rooting rate of shoot could reach 95% in 1/2MS medium supplemented with 0.5 mg · L-1' NAA, so it was optimal medium for rooting. Survival rate of the established plantlets could reach 92% after transferring to growing medium with composition of 50% peat moss, 40% vermiculite and 10% polite. Shoot could root after quickly dipping in 200 g · L"' IBA, and survival rate could reach 60% in the same medium.%为建立高效的花曲柳组培快繁体系,以花曲柳休眠芽萌发获得的带芽茎段和成熟种子无菌萌发获得的带芽茎段为外植体,通过基本培养基和不同植物生长调节剂组合的筛选,诱导出丛生芽,获得了再生植株.结果表明:花曲柳休眠芽采集的最适时期是4月中旬,适合的基本培养基为改良的B5培养基,在改良的B5培养基上添加BA 4.0 mg·L-1和IBA 0.10 mg·L-1,茎芽可以实现增殖,增殖系数可达2.3;在相同培养基上,无菌苗茎段的茎芽增殖系数可达3.0以上；最适的试管内生根培养基为添加NAA 0.5 mg·L-1的1/2MS培养基,茎芽的生根率可达95％,再生植株在草炭土、蛭石和珍珠岩体积比5:4:1的栽培基质中移栽成活率为92％.茎芽速蘸IBA 200 g·L-1后在上述栽培基质中试管外生根,植株再生率可达60％.
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