枣果实中苯丙氨酸解氨酶基因的克隆及序列分析

摘要

In order to provide theoretical basis for researching development mechanism of jujube pit at molecular level and cultivating improved jujube variety without pit, using young fruits from “Jinsixiaozao” as materials, PAL gene was cloned and analyzed by bioinformatics method. A PAL gene was cloned for the first time from jujube, named ZjPAL. The ZjPAL was 1 954 bp and encoded 559 amino acids. The results of multiple alignments showed that ZjPAL had extensive homology with some PAL genes in other plant. The same deamination sites and catalytic active sites in protein coded by ZjPAL were found as those in PAL in Oryza sativa and Zea mays.%为给从分子水平上揭示枣核发育的机理及培育无核枣优良品种提供理论基础,以"金丝小枣"幼果为试材,对枣果实中苯丙氨酸解氨酶(PAL)基因进行了克隆,并进行了生物信息学分析.用分子克隆方法首次从枣树中克隆了苯丙氨酸解氨酶基因部分.DNA序列,并命名为ZjPAL,ZjPAL长1954 by,编码559个氨基酸.通过核苷酸和氨基酸序列多重比对,发现ZjPAL与其它植物的PAL基因高度同源.ZjPAL编码的氨基酸序列包含与水稻、玉米PAL相同的脱氨基位点和催化活性位点.