油茶EMF2基因的全长cDNA克隆及序列分析

摘要

EMF2 gene maintains vegetative development by repressing flowering program,and plays an important role in the process of flower organ development.In order to offer a reference for the researches on epigenetic regulation of Camellia oleifera characters,the full-length cDNA of EMF2 gene in C.oleifera (Huaxin) was cloned and analyzed by 5'RACE and 3'RACE techniques.The results show that the full length of the gene is 2 210 bp,the ORF box included 1 866 bp and encodes 621 amino acids,and it is named Co-EMF2.The results of multiple sequence alignment show that Co-EMF2 has the typical conserved sequences of EMF2 and relevant characters,and it has high homology with EMF2 in other plant species,the protein is a labile and hydrophobic nuclear protein,and its Isoelectric point is 6.17.%EMF2基因是植物营养组织中的主要开花抑制基因,在植物花器官发育过程中发挥重要的作用.为给油茶性状表观遗传学调控研究提供参考依据,以“华鑫”油茶的组培幼苗为试验材料,采用3'RACE和5'RACE技术对油茶EMF2基因进行克隆及序列分析.结果表明:该基因全长2 210 bp,其中开放阅读框为1 866 bp,编码621个氨基酸,该基因被命名为Co-EMF2.多序列比对后发现,Co-EMF2具有EMF2基因特有的保守序列和相关特征,且与其它植物的EMF2基因具有较高的相似性.文中进而对其蛋白质序列和理化性质进行了分析,结果表明:其蛋白质属不稳定蛋白,等电点为6.17,是疏水性蛋白,亚细胞定位预测结果表明其位于细胞核中.