红檵木腋芽高效快繁研究

摘要

为给红檵木组织培养与工厂化育苗提供技术参考，分别以红檵木2年生茎段和当年生茎段为外植体，通过优化红檵木腋芽的丛生芽诱导与增殖、生根与移栽方案，最终建立了以腋芽为基础的红檵木高效快繁体系。结果表明：红檵木茎段腋芽丛生芽诱导与增殖的最佳培养基配方分别为MS＋6-BA 1.3 mg/L＋NAA 0.2 mg/L和MS＋6-BA 1.2 mg/L＋IBA 0.07 mg/L＋Vc 5.0 mg/L；丛生芽生根的最佳培养方案为：选取高度在1.0～3.0 cm之间的植株，将其接种到3/4MS＋IBA 4 mg/L的培养基当中；生根组培苗的最佳移栽方案为：炼苗1周后，将其移栽到珍珠岩∶蛭石∶腐殖土＝1∶1∶3的基质中。%In order to provide a technical reference for tissue culture and factory seedling of Loropetalum chinense var.rubrum, using biennial and annual stems as explants, the high-efifcient and fast propagation system ofL. chinense var.rubrum was established, through optimizing the protocol of induction, multiplication, rooting and transplanting of multiple shoots of axillary bud in L. chinense var.rubrum. The results showed that the optimal mediums for multiple shoots induction and multiplication were MS+6-BA 1.3 mg/L+NAA 0.2 mg/L and MS+6-BA 1.2 mg/L+IBA 0.07 mg/L+Vc 5.0 mg/L, respectively. The optimal program for rooting of multiple shoots was choosing the buds at 1.0-3.0 cm height and inoculating to the medium of 3/4 MS+IBA 4 mg/L. And the optimal program for transplanting of rooting tissue culture seedling was domesticating the buds for a week and then transplanting them into a kind of matrix with perlite, vermiculite and humus soil (1∶1∶3).