目的：建立清热止嚏颗粒中黄芩苷、芍药苷和葛根素含量的测定方法。方法：采用反相高效液相色谱法，色谱柱为Kromasil-C18(150 mm×4.6 mm，5μm)，流动相为乙腈-0.1%磷酸溶液(20：80)，采用等度洗脱，改变体积流量以及波长：在1～12分钟内，流量1.0 mL/min，检测波长230 nm；在12.01～20.0分钟，流量1.5 mL/min，检测波长278 nm；柱温为30℃，进样量20μL。结果：黄芩苷、芍药苷和葛根素线性范围分别为6～100μg/mL、30～350μg/mL、12～150μg/mL；平均回收率分别为100.3%，99.91%和99.93%；RSD为0.54%、0.47%和0.48%。结论：该方法准确、简便、重复性好，可用于清热止嚏颗粒中黄芩苷、芍药苷和葛根素的定量测定。%To establish a method for detection of baisalin, paeoniflorin and puerarin inQingReZhi-Tigranules. Methods: Reversed-Phase High Performance Liquid Chromatography(RP-HPLC) was applied with Kro-masil-C18 as chromatographic column (150 mm×4.6 mm, 5μm) and acetonitrile-0.1% phosphoric acid solution (20:80) as mobile phase. Volume flow and wavelength varied with isocratic elution. Set flow rate as 1.0 mL per minute and wavelength as 230 nm in 1 to 12 minutes; set flow as 1.5 mL per minute and wavelength as 278 nm in 12.01 to 20.0 minutes. The column temperature was set at 30℃and injection volume was 20μL. Results: Linear ranges of baisalin, paeoniflorin and puerarin were 6~100μg/mL, 30~350μg/mL, 12~150μg/mL respectively; averages recov-eries were 100.3%, 99.91% and 99.93%; RSD were 0.54%, 0.47% and 0.48%. Conclusion: The method is accurate, convenient, and reproducible for determination of baisalin, paeoniflorin and puerarin inQingReZhiTigranules.
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