金属栅栏式腭器官培养模型的建立

摘要

目的 建立金属栅栏式小鼠腭器官培养模型.方法 选取妊娠14d的孕鼠20只,采用金属栅栏静式培养法,将腭器官分别培养24和48h,肉眼以及苏木精-伊红染色观察腭器官在体外的发育过程.结果 腭器官发育良好,细胞形态正常.腭器官培养24 h后可见到中脊上皮;培养48 h后可见中脊上皮消失,腭突融合.结论 本方法为研究腭裂发病机制提供了一个良好的体外模型.%Objective The purpose of this study was to establish a palatal organ culture method and to investigate the palatogenesis in vitro. Methods 20 pregnant 14-day mice were killed, embryos were separated ascetically, and palatal shelves were dissected and placed on a modified Trowell's system. All explants were cultured 24 h and 48 h respectively. Finally, all explants were embedded and stained by Hematoxylin and Eosin. Results All explants grew healthy. After incubation for 24 h, medial edge epithelium maintained, whereas after 48 h, medial edge epithelium disappeared, bilateral mesenchymal cells contacted, palates fused. Conclusion This method provides an effective way for investigating the etiology of cleft palate in vitro.