Effects of Methanol on Embryonic Mouse Palate in Serum-Free Organ Culture

摘要

The present study examines the developmental toxicity of methanol in the palateusing a serum-free organ culture model. Gestation day 12 CD-1 mouse embryos were dissected and mid-craniofacial tissues were cultured in BGJ medium at 37 C for 4 days with medium changes at 24 hr intervals. Cultures were exposed to methanol from 0-20 mg/ml for 6 hr, 12 hr, 1 or 4 days. Some cultures were exposed to ethanol for 4 days at doses ranging from 0-15 mg/ml. All cultures were gassed with a 50% O2, 5% CO2, and 45% N2 upon addition of fresh medium and prior to the addition of alcohol. In general the ethanol was more potent than methanol for inhibition of protein and DNA synthesis and palatal fusion. The study demonstrated that methanol can selectively affect specific sensitive cell populations and has effects on proliferation and cell fate.