低强度脉冲超声波联合引导骨组织再生促进牙周骨缺损修复的实验研究

摘要

Objective To study the repair effect of low intensity pulsed ultrasound (LIPUS) irradiating combined with guided bone regeneration (GBR) on the defect of Beagle dog canines periodontal bone. Methods Four canine teeth of every beagle dog (8 beagle dogs) were randomly distributed: Group 1 (LIPUS disposal + GBR+ autogenous bone graft group), group 2 (LIPUS disposal + autogenous bone graft group), group 3 (GBR + autogenous bone graft group), blank control group. The model of periodontal bone defect was established in the 1/3 part of the root buccal area. According to the group division, autogenous bone were grafted, group 1 and group 2 were disposed by LIPUS 20min·d-1. The intensity of ultrasound were 30mW·cm-2. Group 1 and group 3 were injected with Bio-Gide collagen membrane. The beagle dogs were executed at 6 and 8 weeks of the disposal of LIPUS and then Micro-CT test and analysis were conducted to periodontal bone defect area of each group. Results By clinical observation, there were different degrees of shrinkages of the area of periodontal bone defect in each group. The Micro-CT test analysis indicated that there was a statistical difference among the number, the thickness as well as the size of bone trabecula of each grouprn(P<0.05). However, there was no statistical difference (F>0.05) in terms of bone issue measurement index between 6 to 8 weeks of each group. Group 1 had the most new bone. Conclusion LIPUS has the potential to promote the repair of periodontal bone defect. There-fore the combination of LIPUS and GBR may be more conducive to the repair and regeneration of periodontalrnbone defect.%目的 探讨低强度脉冲超声波(LIPUS)联合引导骨组织再生(GBR)对Beagle犬尖牙牙周骨缺损的修复效应.方法 将8只Beagle犬中每只犬的4颗尖牙随机分配到4组:实验1组(LIPUS处理+GBR+自体骨移植组),实验2组(LIPUS处理+自体骨移植组),实验3组(GBR+自体骨移植组),空白对照组.在犬尖牙颊侧区根中1/3处制备牙周骨缺损模型,根据分组,自体骨移植后,实验1组和2组采用LIPUS处理20 min·d-1,超声强度为30 mW·cm-2,实验1、3组植入Bio-Gide胶原膜.在LIPUS处理6、8周后处死Beagle犬,对各组尖牙牙周骨缺损区进行Micro-CT检测和分析.结果 临床观察各组牙周骨缺损面积均不同程度缩小.Micro-CT检测分析表明,各组6周与8周时的骨小梁数目、骨小梁厚度和骨小梁体积均无统计学差异(P＞0.05),而各组之间的骨组织测量指标均有统计学差异(P＜0.05),实验1组新生骨量最多.结论 LIPUS具有促进牙周骨缺损修复的潜能,LIPUS与GBR结合可能更有利于牙周骨缺损的修复与再生.