AFM功能化成像与分子识别研究进展

摘要

In the past decades, AFM have great advances in high resolution molecular imaging and single molecule detection applications. AFM single molecular force 8pectroscopy(SMFS)are available to explore polymer structural changes, molecular identification of bio-signal transmission or protein folding process and unfolding process,the evidence of the relation between structural changes and functions is provided. Some progresses are mentioned such as using AFM in funcuonalization decoration, in locating using SMFS surface protein and polysaccharide and their elasticity. Prospects are also provided concerning future work and difficulties in AFM application.%在过去的几十年间,AFM在高分辨率分子成像与单分子检测上的应用得到了很大进展.AFM单分子力光谱学(SMFS)模式可以用于研究聚合物结构变化,生物信号传导过程的分子识别或蛋白折叠过程.提出了使用AFM进行功能化修饰和SMFS用于定位表面蛋白与多糖,研究表面蛋白的分子延展性的一些进展,并展望了研究前景与难点.