利用Real-time RT-PCR法检测抗病毒活性物质对CMV复制的影响

摘要

To accurately test the content of virus in tobacco after treated by antivirus substance, a real - time PCR assay system was established.A bacteria strain antagonistic to the cucumber mosaic virus (CMV) , B6, was isolated from a field of tobacco.The isolated and purified antivirus substance is referred to as B6p, its relative molecular weight is 40.6 kDa.The virus multiplication characteristics in B6p treated host were analyzed with a round leaf method, and the results showed that B6p inhibited the multiplication of virus in the host, and the multiplication action weakened with the increase of antivirus substance concentration.%为准确检测经活性物质处理后的烟草体内病毒含量,建立了real-time RT-PCR检测体系.从烟田中分离到1株对CMV有拮抗作用的细菌B6,经分离纯化得到拮抗CMV的活性物质B6p,其分子量为40.6 kDa.利用叶圆片法,分析了活性物质处理后寄主体内病毒的复制特点,结果表明寄主体内的病毒复制作用受到了拮抗活性物质的抑制,并且随着拮抗活性物质浓度的增加病毒的复制作用减弱.