靛蓝在虹鳟鱼体内定位分布的放射自显影研究

摘要

In the study,the whole-body kinetics of indigo in rainbow trout was examined through exposure via ambient water.Determined by autoradiography,there was an uptake of14,C-indigo(18,nM)from the water and a subsequent elimina-tion into the bile within 20,mins.At later time-points(3-24,h of exposure;24,h of exposure plus 1-2days in clean water), high concentration14C labelled substances were present in the biliary excretory pathways.A high concentration of radioactivity appeared in the gill filaments throughout the exposure period(24,h),and the concentration gradually decreased when fish were put in clean water for depuration.A selective labelling of the olfactory organ was also observed.The uptake of radioac-tivity in the kidney and liver was pronounced but lower than in the gills.The overall distribution pattern of14,C-indigo was similar in fish exposed to the vehicle(DMSO)and in fish exposed to the CYP1 inducer,PCB126.The accumulation of14,C-indigo in the gills was,however completely blocked in PCB 126-exposed fish exposed to the CYP1 inhibitor ellipticine.%以虹鳟鱼为实验鱼类，通过水浴实验，以CYP1诱导剂(PCB126)为研究对象，分析了靛蓝在鱼全身代谢的动力学情况。通过放射自显影方法并利用放射性14,C 标记的靛蓝(18,nM)进行水浴暴露实验，结果表明：实验开始20,min 后靛蓝消化于胆液中；在随后的实验中(暴露3～24,h)，在胆汁的排泄途径中发现高浓度的标记物。在鱼鳃中也存在高浓度的标记物；换水后，其放射性浓度逐渐降低。同时观察到嗅觉器官中有选择性的标记，肾脏和肝脏中吸收的放射性物质浓度低于鳃。比较暴露于二甲基亚砜(DMSO)和 CYP1诱导物 PCB126的鱼，其14,C-靛蓝总体布局模式相似。CYP1抑制剂玫瑰树碱可以阻拦14,C-靛蓝在鱼鳃中的积蓄。