p38抑制剂对辐射损伤小鼠中免疫细胞的作用

摘要

探讨利用p38抑制剂SB203580(SB)抑制p38通路对6Gy受照小鼠免疫细胞辐射损伤的作用.方法:取雄性C57BL/6小鼠30只,随机分为对照组、照射组和SB组,每组10只.SB组和照射组小鼠接受以6Gy137Csγ射线的全身照射.SB组小鼠照射24 h后腹腔注射SB( 15 mg/kg),每2 d1次,共给药5次,其余2组小鼠腹腔注射对照溶液.小鼠受照10d后处死,取外周血计数,流式细胞仪检测CD4、CD8、B220表达,取骨髓细胞测定ROS水平.结果:照射组外周血白细胞(WBC)、CD8、B220细胞比例较对照组明显下降,骨髓ROS水平升高(P＜0.01).SB组外周血CD8比例较照射组上升,骨髓ROS水平下降(P＜0.01).结论:SB对小鼠6 Gy照射后造血免疫损伤有一定的缓解作用,其机制可能与其降低照射后骨髓细胞ROS水平有关.%Objective:To observe the effects of p38 inhibitor SB2O3580 (SB) on the immune cells in mice exposed to 6 Gy irradiation. MethodS:Thirty C57BL/6 mice were randomly divided into control group, irradiated group and SB group (n~ 10 for each). Mice of control group received sham irradiation, and the rest accepted 6 Gy tolal body irradiation. Mice in SB group were intraperitoneally injected 15 mg/kg of SB every other day for S limes after irradiation. Mice of other two groups were treated with reference solution. Ten days after mice exposed to irradiation, the white blood cells (WBC) were counted. The immune cell phenotypes in peripheral blood were analyzed by flow cytometry. The levels of reactive oxygen species (ROS) of bone marrow cells were detected. Results: Compared to the control group, the proportion of CD8, B220 and WBC were significantly decreased in the irradiated mice, and the ROS level of bone marrow cells were significantly increased (P < 0.01). Compared to the irradiation group, the CD8 ratio was significantly increased in SB group, and the level of ROS in bone marrow cells was significantly decreased in SB group (P < 0.05). Conclusion:The results indicate that p38 inhibitor can protect the immune cells from radiation injury by lowing ROS levels.