miR-224和miR-378e在大肠癌组织中的表达及其临床意义*

摘要

Objective To investigate the expression and clinical significance of microRNA-224 and microRNA-378e in colorectal cancer tissues and normal mucosa adjacent to tumor lesions. Methods The gene chip technology was used to detect the different expression of miRNA in colorectal carcinoma tissues and adjacent normal tissues, which was then confirmed by real-time PCR. The relationship between the pathology and clinical data was analyzed. Results The expres-sion level of miR-224 was significantly up-regulated in tumor tissue, while miR-378e was down-regulated in tumor tissue, which was confirmed by real-time PCR. The expression of miR-224 was strongly associated with histological types, while miR-378e was strongly associated with the infiltration depth of colorectal cancer. Conclusion miR-224 is a potent tumor promoter, while miR-378e is a potent tumor suppressor. Both miR-224 and miR-378e can be used as potential colorectal cancer molecular markers.%　　目的探讨miR-224和miR-378e在原位大肠癌癌组织和癌旁正常黏膜组织中的表达差异，并分析其临床意义。方法 miRNA表达谱芯片技术检测大肠癌癌组织和癌旁组织标本中表达差异的miRNA，运用荧光实时定量聚合酶链反应（real-time PCR）验证其结果，并分析其与临床病理资料之间的关系。结果 miRNA表达芯片分析发现，与正常黏膜组织比较，miR-224在大肠癌组织中表达显著上调，miR-378e在大肠癌组织中表达显著下调，并被real-time PCR所证实。miR-224在不同组织学类型癌组织中表达差异有统计学意义，miR-378e在不同浸润深度癌组织中表达差异有统计学意义，miR-224表达与组织学类型有关，miR-378e表达与大肠癌浸润深度有关。结论miR-224具有潜在的癌基因作用，miR-378e具有潜在的抑癌基因作用，miR-224和miR-378e可作为潜在的大肠癌分子标志物。